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<jats:title>ABSTRACT</jats:title> <jats:p> Recent studies have shown that immune responses against the cell-traversal protein for <jats:named-content content-type="genus-species">Plasmodium</jats:named-content> ookinetes and sporozoites (CelTOS) can inhibit parasite infection. While these studies provide important evidence toward the development of vaccines targeting this protein, it remains unknown whether these responses could engage the <jats:named-content content-type="genus-species">Plasmodium falciparum</jats:named-content> CelTOS <jats:italic>in vivo</jats:italic> . Using a newly developed rodent malaria chimeric parasite expressing the <jats:named-content content-type="genus-species">P. falciparum</jats:named-content> CelTOS (PfCelTOS), we evaluated the protective effect of <jats:italic>in vivo</jats:italic> immune responses elicited by vaccination and assessed the neutralizing capacity of monoclonal antibodies specific against PfCelTOS. Mice immunized with recombinant <jats:named-content content-type="genus-species">P. falciparum</jats:named-content> CelTOS in combination with the glucopyranosyl lipid adjuvant-stable emulsion (GLA-SE) or glucopyranosyl lipid adjuvant-liposome-QS21 (GLA-LSQ) adjuvant system significantly inhibited sporozoite hepatocyte infection. Notably, monoclonal antibodies against PfCelTOS strongly inhibited oocyst development of <jats:named-content content-type="genus-species">P. falciparum</jats:named-content> and <jats:named-content content-type="genus-species">Plasmodium berghei</jats:named-content> expressing PfCelTOS in <jats:named-content content-type="genus-species">Anopheles gambiae</jats:named-content> mosquitoes. Taken together, our results demonstrate that anti-CelTOS responses elicited by vaccination or passive immunization can inhibit sporozoite and ookinete infection and impair vector transmission. </jats:p>

Original publication

DOI

10.1128/iai.00498-16

Type

Journal article

Journal

Infection and Immunity

Publisher

American Society for Microbiology

Publication Date

02/2017

Volume

85