Remarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions
Jönsson P., Southcombe JH., Santos AM., Huo J., Fernandes RA., McColl J., Lever M., Evans EJ., Hudson A., Chang VT., Hanke T., Godkin A., Dunne PD., Horrocks MH., Palayret M., Screaton GR., Petersen J., Rossjohn J., Fugger L., Dushek O., Xu X-N., Davis SJ., Klenerman D.
<jats:p>The αβ T-cell coreceptor CD4 enhances immune responses more than 1 million-fold in some assays, and yet the affinity of CD4 for its ligand, peptide-major histocompatibility class II (pMHC II) on antigen-presenting cells, is so weak that it was previously unquantifiable. Here, we report that a soluble form of CD4 failed to bind detectably to pMHC II in surface plasmon resonance-based assays, establishing a new upper limit for the solution affinity at 2.5 mM. However, when presented multivalently on magnetic beads, soluble CD4 bound pMHC II-expressing B cells, confirming that it is active and allowing mapping of the native coreceptor binding site on pMHC II. Whereas binding was undetectable in solution, the affinity of the CD4/pMHC II interaction could be measured in 2D using CD4- and adhesion molecule-functionalized, supported lipid bilayers, yielding a 2D <jats:italic>K</jats:italic><jats:sub>d</jats:sub> of ∼5,000 molecules/μm<jats:sup>2</jats:sup>. This value is two to three orders of magnitude higher than previously measured 2D <jats:italic>K</jats:italic><jats:sub>d</jats:sub> values for interacting leukocyte surface proteins. Calculations indicated, however, that CD4/pMHC II binding would increase rates of T-cell receptor (TCR) complex phosphorylation by threefold via the recruitment of Lck, with only a small, 2–20% increase in the effective affinity of the TCR for pMHC II. The affinity of CD4/pMHC II therefore seems to be set at a value that increases T-cell sensitivity by enhancing phosphorylation, without compromising ligand discrimination.</jats:p>