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We have used Northern blot analysis to detect mRNA from class I HLA genes in the human choriocarcinoma cell line BeWo, which has been previously shown to express an atypical HLA class I molecule, in the absence of HLA A and B. Hybridization was seen with three class I DNA probes, the strongest being seen with the probe pC800, which corresponds to an 800-bp section of the Cw3 gene. We have made cDNA libraries from BeWo cells and screened for positive clones by using class I DNA probes. Of the clones isolated, we determined the complete sequence of one and partial sequence of five shorter clones. They all code for an identical C locus-related product, which does not correspond to published C locus sequences.


Journal article


Journal of immunology (Baltimore, Md. : 1950)

Publication Date





3281 - 3285


Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Oxford, UK.


Cell Line, Humans, Choriocarcinoma, DNA, DNA Probes, HLA, HLA-C Antigens, Blotting, Northern, Cloning, Molecular, Amino Acid Sequence, Base Sequence, Molecular Sequence Data