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<jats:p>This work demonstrates that with the use of a microfocus synchrotron beam the structure of a novel viral polyhedrin could be successfully determined from microcrystals within cells, removing the preparatory step of sample isolation and maintaining a favourable biological environment. The data obtained are of high quality, comparable to that obtained from isolated crystals, and enabled a facile structure determination. A small but significant difference is observed between the unit-cell parameters and the mosaic spread of<jats:italic>in cellulo</jats:italic>and isolated crystals, suggesting that even these robust crystals are adversely affected by removal from the cell.</jats:p>

Original publication

DOI

10.1107/s1399004714004714

Type

Journal article

Journal

Acta Crystallographica Section D Biological Crystallography

Publisher

International Union of Crystallography (IUCr)

Publication Date

01/05/2014

Volume

70

Pages

1435 - 1441