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<jats:p>Single-wavelength anomalous dispersion of S atoms (S-SAD) is an elegant phasing method to determine crystal structures that does not require heavy-atom incorporation or selenomethionine derivatization. Nevertheless, this technique has been limited by the paucity of the signal at the usual X-ray wavelengths, requiring very accurate measurement of the anomalous differences. Here, the data collection and structure solution of the N-terminal domain of the ectodomain of HCV E1 from crystals that diffracted very weakly is reported. By combining the data from 32 crystals, it was possible to solve the sulfur substructure and calculate initial maps at 7 Å resolution, and after density modication and phase extension using a higher resolution native data set to 3.5 Å resolution model building was achievable.</jats:p>

Original publication

DOI

10.1107/s139900471401339x

Type

Journal article

Journal

Acta Crystallographica Section D Biological Crystallography

Publisher

International Union of Crystallography (IUCr)

Publication Date

01/08/2014

Volume

70

Pages

2197 - 2203