Monoclonal Antibody to Human Platelet Glycoprotein I I. IMMUNOLOGICAL STUDIES
McMichael AJ., Rust NA., Pilch JR., Sochynsky R., Morton J., Mason DY., Ruan C., Tobelem G., Caen J.
Summary. A monoclonal hybridoma antibody specific for platelet glycoprotein I complex is described. The nature of the antigen was determined by demonstration that it was chymotrypsin sensitive and gave a peak at 150000 daltons on SDS‐PAGE after immunoprecipitation. The expression of the antigen is restricted to platelets and megakaryocytes with at least 1.6 × 104 molecules of antigen per platelet. The antibody failed to bind to platelets from patients with Bernard Soulier syndrome, where there is known to be a deficiency of glycoprotein Ib/Is expression. Binding to platelets from patients with Glanzmann's thrombasthenia was normal.