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Single-cell RNA sequencing allows highly detailed profiling of cellular immune responses from limited-volume samples, advancing prospects of a new era of systems immunology. The power of single-cell RNA sequencing offers various opportunities to decipher the immune response to infectious diseases and vaccines. Here, we describe the potential uses of single-cell RNA sequencing methods in prophylactic vaccine development, concentrating on infectious diseases including COVID-19. Using examples from several diseases, we review how single-cell RNA sequencing has been used to evaluate the immunological response to different vaccine platforms and regimens. By highlighting published and unpublished single-cell RNA sequencing studies relevant to vaccinology, we discuss some general considerations how the field could be enriched with the widespread adoption of this technology.

Original publication

DOI

10.1155/2020/8624963

Type

Journal article

Journal

Journal of immunology research

Publication Date

01/2020

Volume

2020

Addresses

The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford OX3 7DQ, UK.

Keywords

Cell Line, Animals, Humans, Pneumonia, Viral, Coronavirus Infections, Disease Models, Animal, RNA, Viral, Viral Vaccines, Drug Evaluation, Preclinical, Immunity, Cellular, Clinical Trials as Topic, Host-Pathogen Interactions, Immunity, Innate, Pandemics, Single-Cell Analysis, Immunogenicity, Vaccine, Betacoronavirus, Vaccinology, RNA-Seq, COVID-19, SARS-CoV-2