Other Seminars

seminar-banner

Mon 7 Jan 2019 from 12:00 to 13:00

Kennedy Institute Seminars

Mind the gut: dietary influences on gut homeostasis and inflammation

Prof. Gitta Stockinger

Brigitta Stockinger obtained her PhD in Biology at the University of Mainz and then did postdoctoral studies in London and Cambridge and Heidelberg. In 1985 she became a member of the Basel Institute for Immunology where she stayed until 1991. In 1991 Gitta became a group leader in the Division of... Read more

Brigitta Stockinger obtained her PhD in Biology at the University of Mainz and then did postdoctoral studies in London and Cambridge and Heidelberg. In 1985 she became a member of the Basel Institute for Immunology where she stayed until 1991. In 1991 Gitta became a group leader in the Division of Molecular Immunology of the Medical Research Council National Institute for Medical Research (now part of the Francis Crick Institute). Her research initially focused on immune tolerance using T cell receptor transgenic mouse models as well as immunological memory of CD4 T cells, their generation and survival and more recently on deciphering the physiological functions of an environmental sensor, the transcription factor aryl hydrocarbon receptor (AHR) in the immune system. Gitta obtained an ERC Advanced Investigator grant in 2009 to study physiological functions of AhR and in 2013 and 2018 was awarded Wellcome Senior Investigator Grant that will continue and expand the investigation of AhR in innate and adaptive immune cells as well as in epithelial cells of the intestinal barrier. She became a Fellow of the Academy of Medical Sciences in 2005, an EMBO fellow in 2008 and a Fellow of the Royal Society in 2013.

Audience: Members of the University only

Organisers: Laura Sánchez Lazo

Tue 8 Jan 2019 from 13:00 to 14:00

WIMM Occasional Seminars

Wed 9 Jan 2019 from 13:30 to 14:30

ARUK Oxford Drug Discovery Institute Seminar Series

Aggregation of tau and alpha-synuclein: towards a therapeutic target in neurodegenerative diseases

Professor Maria Grazia Spillantini FRS

Audience: Members of the University only

Organisers: Kate Humphrey

Wed 9 Jan 2019 from 16:00 to 17:00

BDI seminars

Big Data Institute, Seminar Rooms, Old Road Campus OX3 7LF

Inaugural Lecture - Preventing infections using pathogen genomics and mathematical modelling: HIV and beyond

Professor Christophe Fraser

Audience: Members of the University only

Organisers: Carol Mulligan-John

Thu 10 Jan 2019 from 13:00 to 14:00

Medical Grand Rounds

Cardiology / Acute General Medicine Firm B

Prof Robin Choudhury, Prof Julian Knight

Cardiology: "Coronary disease in diabetes – sweet memories", Prof Robin Choudhury -- Acute General Medicine Firm B: "Time for precision medicine in sepsis?", Prof Julian Knight -- Chair: Prof Chris Conlon

Cardiology: "Coronary disease in diabetes – sweet memories", Prof Robin Choudhury -- Acute General Medicine Firm B: "Time for precision medicine in sepsis?", Prof Julian Knight -- Chair: Prof Chris Conlon

Audience: Members of the University and NHS clinical staff.

Thu 10 Jan 2019 from 16:30 to 18:30

Experimental Medicine TGU Seminars

From Genetics to Clinic in Autoimmune Diabetes

Professor John Todd

Audience: Members of the University only

Organisers: Professor Holm Uhlig

Fri 11 Jan 2019 from 11:00 to 12:00

Jenner Seminars

Controlling two helminth infections, cysticercosis and echinococcosis, using highly effective recombinant antigen vaccines

Prof Marshall Lightowlers

Audience: Public

Organisers: Lisbeth Soederberg

The seminar will be followed by a buffet lunch in the Doll Building Atrium

Mon 14 Jan 2019 from 12:00 to 13:00

Kennedy Institute Seminars

Regulation of B cell responses in sterile inflammation

Prof Mikael Karlsson

Audience: Members of the University only

Organisers: Laura Sánchez Lazo

Mon 14 Jan 2019 from 13:00 to 14:00

WIMM MONDAY SEMINARS

Reconstructing the immune system using single cell RNA sequencing

Prof Muzlifah Haniffa

Audience: Members of the University only

Organisers: Liz Cloke

Tue 15 Jan 2019 from 13:00 to 14:00

Molecular Haematology Unit, WIMM

MHU Student Presentations

Audience: Members of the University only

Organisers: Liz Rose

Tue 15 Jan 2019 from 13:00 to 14:00

Population Health Seminars

Richard Doll Seminar: A genetic risk score to guide personalised, age-specific prostate cancer screening

Dr Tyler Seibert

Audience: Members of the University only

Organisers: Graham Bagley

Wed 16 Jan 2019 from 13:30 to 14:30

MRC HIU Wednesday Seminar Series

Molecular archaeology of cancer

Dr Peter Van Loo

Audience: Members of the University only

Organisers: Anne Farmer

Wed 16 Jan 2019 from 13:30 to 15:00

Ethox Centre and Wellcome Centre for Ethics and Humanities

Big Data Institute, Seminar Room 1, Old Road Campus OX3 7LF

Thu 17 Jan 2019 from 11:00 to 12:00

Ludwig Institute Seminar Series

Understanding the immune response to persistent human T-cell leukaemia virus (HTLV-I) infections (exact title tbc)

Professor Charles Bangham

Audience: Members of the University only

Organisers: Christina Woodward

Thu 17 Jan 2019 from 13:00 to 14:00

Medical Grand Rounds

AICU / Oncology

Dr Benjamin Fairfax, Dr Chris Andersen

AICU: "What Becomes of the Broken Hearted?", Dr Chris Andersen -- Oncology: "Using peripheral blood to predict clinical outcomes from immunotherapy for metastatic melanoma", Dr Benjamin Fairfax -- Chair: Prof Chris Conlon

AICU: "What Becomes of the Broken Hearted?", Dr Chris Andersen -- Oncology: "Using peripheral blood to predict clinical outcomes from immunotherapy for metastatic melanoma", Dr Benjamin Fairfax -- Chair: Prof Chris Conlon

Audience: Members of the University and NHS clinical staff.

Thu 17 Jan 2019 from 14:30 to 15:30

Experimental Medicine TGU Seminars

Enteric viral infection in childhood and coeliac disease

Dr Michael Fitzpatrick

Coeliac disease is a common immune condition where susceptible individuals develop inflammation of the gut in response to gluten, a protein in wheat. Whilst genetics play an important role in the development of coeliac disease, there is evidence that an environmental trigger is required for coeliac... Read more

Coeliac disease is a common immune condition where susceptible individuals develop inflammation of the gut in response to gluten, a protein in wheat. Whilst genetics play an important role in the development of coeliac disease, there is evidence that an environmental trigger is required for coeliac disease to develop. Circumstantial evidence has suggested that viral infections in childhood could be that trigger, but definitive proof remains elusive. A recent study has suggested that reovirus, a virus affecting the gut that generally causes no symptoms, can trigger a condition like coeliac disease in mice under experimental conditions, however it remains unclear if this infection is associated with coeliac disease in humans. The discovery of infections that can trigger coeliac disease could have a profound impact on the prevention of the development of coeliac disease, as well as on the prevention of diseases associated with it, such as Type 1 Diabetes. We will test the association between reovirus, along with a number of other viral infections that affect the gut, and the development of coeliac disease by using stored samples from a recent study of diagnostic methods of coeliac disease in children. This large, well-described, cohort of children with and without coeliac disease provides an ideal group in which to test for an association between viral infection and coeliac disease. We will perform tests to measure antibody responses to these viruses in children with and without coeliac disease.

Audience: Members of the University only

Organisers: Dr Carolina Arancibia

Thu 17 Jan 2019 from 15:30 to 16:30

Experimental Medicine TGU Seminars

An investigation into the stability of the adaptive and innate immune compartments within a transplanted small bowel: A pilot study

Dr Philip Allan

Intestinal failure, where the gut fails to absorb sufficient nutrients, is a debilitating condition which requires treatment with intravenous parenteral nutrition. Unfortunately, this treatment can be complicated by severe infections and venous blood clots, and in such circumstances intestinal... Read more

Intestinal failure, where the gut fails to absorb sufficient nutrients, is a debilitating condition which requires treatment with intravenous parenteral nutrition. Unfortunately, this treatment can be complicated by severe infections and venous blood clots, and in such circumstances intestinal transplantation can be an effective treatment. However intestinal transplantation suffers from high rates of graft rejection, as well as cases of graft versus host disease (GVHD) and has only a 60% 5-year survival. Large numbers of immune cells from the donor are transferred into an intestinal transplant, and the interactions of the immune cells from donor and recipient, the survival of donor immune cells in the transplant, and the mechanisms that allow colonisation of the transplanted tissue by recipient immune cells are not well understood. Our work will study these questions through studying intestinal immune cell populations in recipients of intestinal transplants at the Oxford University Hospitals NHS Foundation Trust transplantation unit, one of only two centres offering adult intestinal transplantation in the United Kingdom. These results will have direct patient benefit by elucidating the mechanisms that drive graft rejection and GVHD in intestinal transplantation, and by identifying strategies that might prevent these complications. Moreover, these results may provide insights into more fundamental questions in human immunology, including the dynamics of tissue residency of immune cells in the gut.

Audience: Members of the University only

Organisers: Dr Carolina Arancibia

Fri 18 Jan 2019 from 08:00 to 09:00

Surgical Grand Rounds

Communication in Healthcare: A Failure in Need of Rescue?

Professor Amir A. Ghaferi

Audience: Members of the University only

Organisers: Tarryn Ching

Fri 18 Jan 2019 from 09:15 to 10:15

MRC HIU Friday Morning Lab Meetings

An essential role for the Zn2+ transporter ZIP7 in B cell development

Professor Richard Cornall

Audience: Members of the University only

Organisers: Anne Farmer

Fri 18 Jan 2019 from 11:00 to 12:00

Strubi seminars

Probing the mechanism of the SAMHD1 HIV-1 restriction factor

Dr Ian Taylor

SAMHD1 is a post-entry cellular restriction factor that inhibits HIV-1 replication in myeloid-lineage and resting CD4+ T cells. The mechanism of SAMHD1 restriction has been disputed but the predominant theory is that SAMHD1 dNTP triphosphohydrolase activity blocks HIV-1 infection by reducing the... Read more

SAMHD1 is a post-entry cellular restriction factor that inhibits HIV-1 replication in myeloid-lineage and resting CD4+ T cells. The mechanism of SAMHD1 restriction has been disputed but the predominant theory is that SAMHD1 dNTP triphosphohydrolase activity blocks HIV-1 infection by reducing the cellular dNTP pool to a level that does not support viral reverse transcription. A large body of structural and biochemical studies have demonstrated that the active form of SAMHD1 is a protein tetramer that contains four regulatory allosteric sites each accommodating a deoxynucleotide/nucleotide pair and four active sites that hydrolyse the dNTP substrates. In addition, other studies have shown that the dNTP triphosphohydrolysis reaction is regulated by tetramer stability, controlled by SAMHD1 phosphorylation at residue T592. However, although, this wealth of information has contributed significantly to our understanding of SAMHD1 restriction, regulation and activation the exact nature of SAMHD1 cellular activity that restricts HIV-1 and the molecular details catalytic mechanism of dNTP hydrolysis have remained unclear. Therefore, to elucidate the molecular mechanism of dNTP triphospho-hydrolysis by SAMHD1, we have undertaken virological studies together with comprehensive, enzymological studies employing deoxynucleotide substrate and activator analogues and determined crystal structures of catalytically active SAMHD1 with dNTP-mimicking, competitive inhibitors. The SAMHD1-inhibitor co-crystal structures show in atomic detail how dNTP substrates are coordinated at the SAMHD1 active site and reveal how the activated protein cleaves the phospho-ester bond in the dNTP substrate. In conclusion, these studies now clarify the anti-HIV-1 activity of SAMHD1 and provide the molecular details of the SAMHD1 reaction mechanism demonstrating how dNTP substrates are hydrolysed and enable more accurate prediction of whether new and existing antiviral and anticancer drugs are hydrolysed by SAMHD1.

Audience: Members of the University only

Organisers: Agata Krupa

Fri 18 Jan 2019 from 11:00 to 12:00

MRC HIU Wednesday Seminar Series

Accessories to the crime: functions of immune cells in the tumor microenvironment

Dr Arianna Calcinotto

Audience: Members of the University only

Organisers: Anne Farmer

Fri 18 Jan 2019 from 13:00 to 14:00

DPAG Head of Department Seminar Series

Adipose tissue expandability, lipotoxicity and the metabolic syndrome

Professor Antonio Vidal-Puig

The link between obesity and type 2 diabetes is clear on an epidemiological level, however the mechanism linking these two common disorders is not well defined. One hypothesis linking obesity to type 2 diabetes is the adipose tissue expandability hypothesis. The adipose tissue expandability... Read more

The link between obesity and type 2 diabetes is clear on an epidemiological level, however the mechanism linking these two common disorders is not well defined. One hypothesis linking obesity to type 2 diabetes is the adipose tissue expandability hypothesis. The adipose tissue expandability hypothesis states that a failure in the capacity for adipose tissue expansion, rather than obesity per se is the key factor linking positive energy balance and type 2 diabetes. All individuals possess a maximum capacity for adipose expansion which is determined by both genetic and environmental factors. Once the adipose tissue expansion limit is reached, adipose tissue ceases to store energy efficiently and lipids begin to accumulate in other tissues. Ectopic lipid accumulation in non-adipocyte cells causes lipotoxic insults including insulin resistance, apoptosis and inflammation. This article discusses the links between adipokines, inflammation, adipose tissue expandability and lipotoxicity. Finally, we will discuss how considering the concept of allostasis may enable a better understanding of how diabetes develops and allow the rational design of new anti diabetic treatments.

Audience: Members of the University only

Fri 18 Jan 2019 from 13:00 to 14:00

NDM Seminar Series

The genomics of cancer evolution and metastasis & The role of common genetic variants in cancer gene regulation

Dr David Wedge, Annabelle Lewis

Audience: Members of the University only

Organisers: Kathryn Smith

Mon 21 Jan 2019 from 12:00 to 13:00

Kennedy Institute Seminars

Circadian clock regulation of mucosal immunity

Professor David Ray

Recent studies reveal that airway epithelial cells are critical pulmonary circadian pacemaker cells, mediating rhythmic inflammatory responses. Using mouse models we now identify the rhythmic circadian repressor REV-ERB as essential to the mechanism coupling the pulmonary clock to innate... Read more

Recent studies reveal that airway epithelial cells are critical pulmonary circadian pacemaker cells, mediating rhythmic inflammatory responses. Using mouse models we now identify the rhythmic circadian repressor REV-ERB as essential to the mechanism coupling the pulmonary clock to innate immunity. Dual mutation of REV-ERBα and its paralog REV-ERBβ in bronchial epithelia further augmented inflammatory responses and chemokine activation, but also initiated a basal inflammatory state, revealing a critical homeostatic role for REV-ERB proteins in the suppression of the endogenous pro-inflammatory mechanism in un-challenged cells. Thus, dynamic changes in stability of REV-ERB protein couple the core clock to innate immunity. ---- David trained in general internal medicine and endocrinology in the UK, and California. He developed a research interest in nuclear receptor function in inflammation, which was supported by MRC, and GSK fellowships. He then identified the importance of the circadian clock machinery in regulating innate immunity, using lung, joint and gut models, and is extending these studies to human cohorts. He recently moved to Oxford, with Wellcome and MRC support, to work on circadian control of inflammation in the lung, and the re-wiring of circadian metabolism by chronic inflammatory processes.

Audience: Members of the University only

Organisers: Laura Sánchez Lazo

Mon 21 Jan 2019 from 13:00 to 14:00

WIMM Science Career Seminars

CAREER SEMINAR: “Tales from our WIMM Job Club – University Teaching and Analytical Support to Industry”

Lucy Eddowes, Emma Jones

This month's seminar series will feature two great speakers. Dr. Emma Jones will talk to us about her work as a University lecturer and Dr. Lucy Eddowes will tell us about working in analytical support to the biomedical industry. Lucy and Emma will also tell us about the "Job Club" they set up... Read more

This month's seminar series will feature two great speakers. Dr. Emma Jones will talk to us about her work as a University lecturer and Dr. Lucy Eddowes will tell us about working in analytical support to the biomedical industry. Lucy and Emma will also tell us about the "Job Club" they set up whilst Postdocs at the WIMM, where they met regularly to discuss ideas and give each other feedback on their plans for next steps after they left the WIMM.

Audience: Members of the University only

Organisers: Natalia Sampaio

Tue 22 Jan 2019 from 12:00 to 13:00

CNCB Seminar Series

Mind the Gap: Super-resolution Imaging of the Extracellular Space of the Brain

Valentin Nagerl

The advent of super-resolution microscopy has created unprecedented opportunities to study the mammalian central nervous system, which is dominated by anatomical structures whose nanoscale dimensions critically influence their biophysical properties. I will present our recent methodological... Read more

The advent of super-resolution microscopy has created unprecedented opportunities to study the mammalian central nervous system, which is dominated by anatomical structures whose nanoscale dimensions critically influence their biophysical properties. I will present our recent methodological advances 1) to analyze dendritic spines in the hippocampus in vivo and 2) to visualize the extracellular space (ECS) of the brain. Using a two-photon–STED microscope equipped with a long working distance objective and ‘hippocampal window’ to reach this deeply embedded structure, we measured the density and turnover of spines on CA1 pyramidal neurons. Spine density was two times higher than reported by conventional two-photon microscopy; around 40% of all spines turned over within 4 days. A combination of 3D-STED microscopy and fluorescent labeling of the extracellular fluid allows super-resolution shadow imaging (SUSHI) of the ECS in living brain slices. SUSHI enables quantitative analyses of ECS structure and produces sharp negative images of all cellular structures, providing an unbiased view of unlabeled brain cells in live tissue.

Audience: Members of the University only

Organisers: Fiona Woods

Tue 22 Jan 2019 from 13:00 to 14:00

Molecular Haematology Unit, WIMM

The human centromere, a paradigm in chromatin-based epigenetic inheritance

Dr Lars Jansen

Audience: Members of the University only

Organisers: Liz Rose

Tue 22 Jan 2019 from 13:00 to 14:00

Population Health Seminars

Richard Doll Seminar: Sustainable and healthy food systems - Now and in the future

Professor Alan Dangour

Audience: Members of the University only

Organisers: Graham Bagley

Thu 24 Jan 2019 from 13:00 to 14:00

Medical Grand Rounds

Horton Hospital / Rheumatology

Dr Lorraine O’Neill, Dr Alex Mentzer, Dr Ian Arnold, Dr Neil Stewart

Horton Hospital: "Stroke: The Heart of the Matter", Dr Alex Mentzer, Dr Ian Arnold and Dr Neil Stewart -- Rheumatology: "A Wolf in Sheep's Clothing", Dr Lorraine O’Neill -- Chair: Prof Chris Conlon

Horton Hospital: "Stroke: The Heart of the Matter", Dr Alex Mentzer, Dr Ian Arnold and Dr Neil Stewart -- Rheumatology: "A Wolf in Sheep's Clothing", Dr Lorraine O’Neill -- Chair: Prof Chris Conlon

Audience: Members of the University and NHS clinical staff.

Fri 25 Jan 2019 from 08:00 to 09:00

Surgical Grand Rounds

Novel methods for predicting growth of AAAs in humans – an update from the OxAAA study

Dr Regent Lee

Audience: Members of the University only

Organisers: Tarryn Ching

Fri 25 Jan 2019 from 11:00 to 12:00

Strubi seminars

"Cell shape formation controlled by cytoskeleton"

Dr Naoko Mizuno

Neurons are highly polarized cells whose shape is controlled by cytoskeleton networks. The formation of neuronal protrusions such as dendrites and axons is mediated by the dynamic nature of microtubules, and it is the basis of neuronal development. Particularly at axon branches, signaling processes... Read more

Neurons are highly polarized cells whose shape is controlled by cytoskeleton networks. The formation of neuronal protrusions such as dendrites and axons is mediated by the dynamic nature of microtubules, and it is the basis of neuronal development. Particularly at axon branches, signaling processes trigger actin re-formation leading to the recruitment of microtubules to reinforce the branching site; however, little is known about this remodeling mechanism. Combining the interdisciplinary methods of cryo-EM, biophysics, and cell biology, we focus on elucidating the mechanism of neuronal cell shape formation and accompanying cytoskeleton remodeling. We will present our recent discovery of a novel factor SSNA1, promoting axon branch formation. To understand the underlying mechanism of branch promotion, we have characterized the interaction of the protein with tubulin and reconstituted its microtubule nucleation process in vitro. Moreover, cryo-EM revealed the surprising observation that SSNA1 facilitates direct microtubule branching. Mutagenesis experiments in primary neurons correlate the molecular remodeling activity with the formation of axon branches.

Audience: Members of the University only

Organisers: Agata Krupa

Fri 25 Jan 2019 from 13:00 to 14:00

DPAG Head of Department Seminar Series

An architectonic type principle integrates cerebral cortical architecture and connectivity

Prof. Dr Claus Hilgetag

The connections that link neurons within as well as between cerebral cortical areas form a multi-scale structural network for communication in the brain. Which principles underlie the organisation of this complex network? We addressed this question by systematically investigating the relation of... Read more

The connections that link neurons within as well as between cerebral cortical areas form a multi-scale structural network for communication in the brain. Which principles underlie the organisation of this complex network? We addressed this question by systematically investigating the relation of essential features of cortico-cortical connections, such as their presence or absence as well as patterns of laminar projection origins and terminations, to fundamental structural parameters of cortical areas, such as their distance, similarity in cortical cytoarchitecture as defined by cortical lamination or neuronal density, and similarity in further macroscopic and microscopic morphological features. These systematic analyses demonstrate the presence of an architectural type principle. Across different species (mouse, cat, macaque monkey and human) and different cortices, the essential features of cortico-cortical connections vary consistently and strongly with the cytoarchitectonic similarity of cortical areas. By contrast, such relations were not found as consistently in multivariate analyses for distance, similarity of cortical thickness or cellular morphological features. The presence of the architectonic type principle across mammalian brains allows direct cross-species predictions of the existence and laminar patterns of projections, including for the human brain, where such data are not directly available experimentally. Moreover, intrinsic brain architecture as characterised by architectural type and neural density also accounts for cellular neuronal features, such as cell size or shape. Thus, these findings illuminate a general principle of neural wiring and integrate cortical connectivity and architecture across scales of organisation, with implications for models of cortical physiology as well as developmental mechanisms.

Audience: Members of the University only

Mon 28 Jan 2019 from 11:00 to 12:00

Department of Oncology

Publishing and Careers at Nature Research

Dr Anne Mirabella

Audience: Members of the University only

Organisers: Amanda O'Neill

Mon 28 Jan 2019 from 12:00 to 13:00

Kennedy Institute Seminars

Understanding CTL: genes, membranes and immunodeficiencies

Prof Gillian Griffiths

Audience: Members of the University only

Organisers: Laura Sánchez Lazo

Mon 28 Jan 2019 from 13:30 to 16:30

Tropical Medicine Seminars

Oxford Union - Goodman Library

Public debate at The Oxford Union: Climate Change and Health

Embedded within the Centre for Tropical Medicine and Global Health, the MSc IHTM is a multidisciplinary and interdisciplinary programme examining major challenges to the health of populations in resource-limited contexts. We expect our students to be future leaders in global health, either in governments, non-governmental organisations and/or academic institutions.

Embedded within the Centre for Tropical Medicine and Global Health, the MSc IHTM is a multidisciplinary and interdisciplinary programme examining major challenges to the health of populations in resource-limited contexts. We expect our students to be future leaders in global health, either in governments, non-governmental organisations and/or academic institutions.

Audience: Public

Organisers: Sarah Jones

Students from the MSc International Health & Tropical Medicine class will debate issues surrounding climate change and health (three debates during the course of the afternoon). This year’s cohort are a diverse group of 26 students from 21 countries with varied disciplinary backgrounds. All welcome!

Mon 28 Jan 2019 from 14:00 to 15:00

BDI seminars

Big Data Institute, Seminar room 0, Old Road Campus OX3 7LF

CKB Seminar: Using genetics to explore the consequences of obesity

Dr Jess Tyrrell

Audience: Members of the University only

Organisers: Graham Bagley

Tue 29 Jan 2019 from 13:00 to 14:00

Population Health Seminars

Richard Doll Seminar: Incorporating biomarkers into breast cancer trials - where are we at?

Audience: Members of the University only

Organisers: Graham Bagley

Wed 30 Jan 2019 from 13:30 to 14:30

MRC HIU Wednesday Seminar Series

Uncovering the enigmatic role of IL-33 in GI inflammation and cancer

Professor Theresa Pizarro

Audience: Members of the University only

Organisers: Anne Farmer

Thu 31 Jan 2019 from 11:00 to 12:00

Strubi seminars

"CHEMICAL-GENETIC HYBRID BIOSENSORS FOR FLUORESCENCE MICROSCOPY"

Dr Alison G. Tebo

Fluorescence imaging has become an indispensable tool in cell and molecular biology. Genetically encoded fluorophores have revolutionized fluorescence microscopy, giving experimenters exquisite control over the localization and specificity of tagged constructs. However, these systems present... Read more

Fluorescence imaging has become an indispensable tool in cell and molecular biology. Genetically encoded fluorophores have revolutionized fluorescence microscopy, giving experimenters exquisite control over the localization and specificity of tagged constructs. However, these systems present certain drawbacks and as such, alternative hybrid systems based on the interaction between a small molecule fluorophore and a protein have been developed. A way to avoid unspecific background in cells and achieve high imaging contrast is to use fluorescent probes that display no fluorescence until labeling occurs. Such probes are often called fluorogenic probes to highlight their ability to generate fluorescence upon reaction/interaction with their target. A new fluorogenic hybrid system called the Fluorescence-activation and Absorption Shifting Tag (FAST) was developed, which consists of a small protein tag that reversibly and dynamically binds a small molecule chromophore, activating its fluorescence. Hybrid chemical-genetic systems such as FAST represent unique opportunities to extend the utility of the system as the protein and fluorogen present two opportunities for engineering. I will present work on improvements to the system and the development of biosensors based on such hybrid systems. 1.Plamont, M.-A. et al. Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo. Proc. Natl. Acad. Sci. U. S. A 113, 497–502 (2016). 2.Tebo, A. G., Pimenta, F. M., Zhang, Y. & Gautier, A. Improved Chemical-Genetic Fluorescent Markers for Live Cell Microscopy. Biochemistry 57, 5648–5653 (2018). 3.Tebo, A. G. et al. Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors. ACS Chem. Biol. 13, 2392–2397 (2018).

Audience: Members of the University only

Organisers: Agata Krupa

Thu 31 Jan 2019 from 17:00 to 18:00

Population Health Seminars

Inaugural Lecture - Clinical Trials: Trying to work out who benefits

Professor Robert Hills

Audience: Members of the University only

Organisers: Graham Bagley