Flow Cytometry Facility

Contact: Mr Andrew Worth, Flow Sorting Facility Manager
Tel: +44 (0)1865 617640
Email: andrew.worth@ndm.ox.ac.uk

Background

Flow cytometry allows the simultaneous measurement of certain characteristics of individual cells based on light signals generated when fluorescently labelled cells are made to flow past one or more laser beams. Prime examples of cell characteristics that can be analysed include size, granularity, phenotypic markers, intracellular proteins, DNA content and calcium flux. Flow sorting extends flow cytometry to the collection of subsets of a mixed cell population, in a purified form, by the electrostatic deflection of droplets containing target cells away from the main flow stream and into a collection tube.

The Flow Cytometry Lab has been set up as a Small Research Facility that is part of the Jenner Institute, in the Old Road Campus Research Building, Oxford.

Services provided

Quantum_Dots

The facility provides two main flow cytometer based services: flow analysis and flow sorting. These services are available to all members of Oxford University, other academic institutions, and industry.

Training and/or experimental and technical assistance is provided as necessary. On-site data analysis may be performed with FlowJo, FACSDiva or Summit.

For flow analysis there are 2 digital cytometers and an old analogue cytometer available for booking. The user has the option of having the samples run for them, in which case they will need to liaise with the facility manager, or they can run their own samples after suitable training.

For cell sorting there is a single high-speed cell-sorter that has a dedicated operator to run the user’s samples. Up to 4 populations can be sorted simultaneously or there is the option to sort into multi-well plates. In all cases the user prepares their own samples.

Facility equipment

Becton Dickinson LSR II (purchased 2007)

LSRII

15-colour digital flow analyser

 

Available parameters (most signals may be defined as linear peak, log peak or area):

 

Beckman Coulter CyAn™ ADP LX High-Performance Research Flow Cytometer (purchased 2005)

9-colour digital flow analyser

Available parameters (most signals may be defined as linear peak, log peak or area):

 

Beckman Coulter Legacy MoFlo MLS High Speed Cell Sorter

MoFlo

7-colour digital fluorescence activated high-speed cell sorter (purchased 2005)

Available parameters (most signals may be defined as linear peak, log peak or area):

 

 

We welcome opportunities for collaboration or business partnership; enquiries can be directed to Mr Andrew Worth:
Email: andrew.worth@ndm.ox.ac.uk
Tel: +44 (0)1865 617640