||Address:||The Jenner Institute, Old Road Campus Research Building
Roosevelt Drive, Oxford, OX3 7DQ
|Tel:||+44 (0)1865 617632|
|DPhil:||Using vaccine-induced human monoclonal antibodies to inform the rational design of an improved PfRH5-based blood-stage vaccine immunogen|
I am a third year DPhil student having joined the Jenner Institute in November 2012 after completing a master’s degree in immunology and allergy at the University of Nottingham and an undergraduate degree in cell biology and physiology at the University of Strasbourg.
The focus of my project is to isolate monoclonal antibodies from PfRH5-vaccinated volunteers and use them to define the most immunologically relevant epitopes of this leading blood-stage vaccine candidate using various complementary biophysical and immunological techniques. This work should lead to the rational design of a second-generation PfRH5-based blood-stage vaccine and/or monoclonal based therapeutic.
Alexander D. Douglas1, , , G. Christian Baldeviano2, Carmen M. Lucas2, Luis A. Lugo-Roman2, Cécile Crosnier3, S. Josefin Bartholdson3, Ababacar Diouf4, Kazutoyo Miura4, Lynn E. Lambert5, Julio A. Ventocilla2, Karina P. Leiva2, Kathryn H. Milne1, Joseph J. Illingworth1, Alexandra J. Spencer1, Kathryn A. Hjerrild1, Daniel G.W. Alanine1, Alison V. Turner1, Jeromy T. Moorhead2, Kimberly A. Edgel2, Yimin Wu5, Carole A. Long4, Gavin J. Wright3, Andrés G. Lescano2, Simon J. Draper1 "Neutralization of Plasmodium falciparum merozoites by antibodies against PfRH5." The Journal of Immunology 192, no. 1 (2014): 245-258.
Alexander D. Douglas*, Andrew R. Williams*,1, Ellen Knuepfer†, Joseph J. Illingworth*, Julie M. Furze*, Cécile Crosnier‡, Prateek Choudhary*, Leyla Y. Bustamante‡, Sara E. Zakutansky*, Dennis K. Awuah*, Daniel G. W. Alanine*, Michel Theron‡, Andrew Worth*, Richard Shimkets§, Julian C. Rayner‡, Anthony A. Holder†, Gavin J. Wright‡ and Simon J. Draper* "A PfRH5-based vaccine is efficacious against heterologous strain blood-stage Plasmodium falciparum infection in aotus monkeys." Cell host & microbe 17, no. 1 (2015): 130-139.
Susanne H Hodgson1,2, Prateek Choudhary1, Sean C Elias1, Kathryn H Milne1, Thomas W Rampling1,2, Sumi Biswas1, Ian D Poulton2, Kazutoyo Miura3, Alexander D Douglas1, Daniel GW Alanine1, Joseph J Illingworth1, Simone C de Cassan1, Daming Zhu3, Alfredo Nicosia4,5,6, Carole A Long3, Sarah Moyle7, Eleanor Berrie7, Alison M Lawrie2, Yimin Wu8, Ruth D Ellis8, Adrian V S Hill1 and Simon J Draper1 "Combining Viral Vectored and Protein-in-adjuvant Vaccines Against the Blood-stage Malaria Antigen AMA1: Report on a Phase 1a Clinical Trial." Molecular Therapy (2014).
Wan, Daniel(co), Fernanda Ludolf(co), Daniel GW Alanine, Owen Stretton, Eman Ali Ali, Nafal Al-Barwary, Xiaowei Wang et al. "Use of humanised rat basophilic leukaemia cell line RS-ATL8 for the assessment of allergenicity of Schistosoma mansoni proteins." (2014): e3124.